Chx-chase assay
WebSep 11, 2024 · CHX pulse-chase assay was performed to analyze the stability of endogenous SNAI2 protein. (E) Quantification of endogenous SNAI2 protein levels presented in D using ImageJ software. 5 Supplemental Figure S4. ASB13 is a major E3 ligase affecting endogenous SNAI2 protein degradation. WebJan 9, 2024 · Cycloheximide (CHX) chase assay. Cycloheximide (CHX) is a protein synthesis inhibitor in eukaryotes, which is used in cell biology to determine the half-life of a given protein [36, 37]. FLO1 cells with IGFBP2 knockdown and control cells were treated with or without ABS for 20 min and then recovered in full medium with 50 μg/ml CHX.
Chx-chase assay
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WebMay 19, 2024 · 蛋白半衰期实验出图如下，这个实验是怎么做的咩。我么你可以把这个实验看成是一个蛋白降解的ic50实验。这个实验只需要用到一个叫放线菌酮（chx）的试剂，就能简单的解释一下为什么蛋白表达有差异。 WebCycloheximide can be used as an experimental tool in molecular biology to determine the half-life of a protein. Treating cells with cycloheximide in a time-course experiment followed by western blotting of the cell lysates for the protein of interest can show differences in protein half-life.
Web1. 100 mg/ml cycloheximide (CHX) stock solution (Sigma-Aldrich, catalog number: C7698) ... μg/ml cyclcoheximide does not cause apoptosis within the time course of the chase assay. It is recommended to start a cell line test with different concentrations of cycloheximide (ranging from 50300 μg/ml) for a leas- t 8-h chase. ... WebApr 10, 2024 · Cycloheximide (CHX) chase assay showed that NC shortened the half-life of ABCB6 protein, indicating the facilitation of its protein degradation by NC. The degradation mechanism of ABCB6 were tested by inhibitor assay, MG132 and CQ is a well-known proteasome inhibitor or autophagy inhibitor [47, 48], respectively. The pretreatment with …
WebChx Chase Assay, supplied by Selleck Chemicals, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more WebJul 23, 2024 · In this study, we performed a cycloheximide (CHX) chase assay to measure the half-lives of approximately 30 human lysosomal membrane proteins (LMPs) and identified RNF152 and LAPTM4A as short-lived membrane proteins. The degradation of both proteins is ubiquitin dependent.
WebSep 7, 2010 · CHX files are used for auditing drawings and reporting any violations in an HTML report. They are particularly useful in situations where multiple parties have …
WebApr 13, 2024 · This effect was confirmed by CHX chase assay. As shown in Fig. 3f–i , when protein synthesis was inhibited by CHX, overexpression of USP10 significantly prolonged the half-life of CCND3 in both ... theoretical discussions definitionWebDetermine the time course of cycloheximide chase beforehand. There can be great variation among proteins. For an unfamiliar protein, it is recommended to start with a 4-hour … theoretical dissertation methodologyWebJan 1, 2015 · 100 mg/ml cycloheximide (CHX) stock solution (Sigma-Aldrich, catalog number: C7698) ... For CL1-5 cells, 300 µg/ml cyclcoheximide does not cause apoptosis … theoretical dissertationWebFIGURE 1 Development of dual-reporter cell lines and a CHX chase assay to monitor UPS function. A, HeLa cells stably expressing Ub G76V-GFP … theoretical discussionsWebMay 13, 2011 · Development of dual-reporter cell lines and a CHX chase assay to monitor UPS function. A, HeLa cells stably expressing Ub G76V-GFP and ODD-Luc were treated with the indicated amounts of MG132 for 2 h. Signal intensities for GFP and luciferase accumulation were normalized to values obtained with DMSO-treated cells. B, ... theoretical dissolved oxygen tableWebApr 1, 2024 · CHX-chase assay Transfected HEK 293T cells were cultured in presence of 50 μg/ml CHX (Sigma), in some instances in combination with 50 μM MG132 (Calbiochem) for the indicated time periods. theoretical distribution ca foundationWebOpening a Chase Secure Banking account comes with a $100 cash bonus when you use the card for 10 purchases within 60 days. Account approval is immediate and you’ll … theoretical dissertation structure