WebTypes of Cloning Vector Cosmid Plasmid Bacteriophage BAC (Bacterial Artificial Chromosome) HAC (Human Artificial Chromosome) YAC (Yeast Artificial Chromosome) Extended Reading: Cloning Vectors Plasmid Vector The plasmid is a type of vector commonly used in recombinant DNA technology. A cosmid is a type of hybrid plasmid that contains a Lambda phage cos sequence. They are often used as a cloning vector in genetic engineering. Cosmids can be used to build genomic libraries. They were first described by Collins and Hohn in 1978. Cosmids can contain 37 to 52 (normally 45) kb of DNA, … See more Cos sequences are ~200 base pairs long and essential for packaging. They contain a cosN site where DNA is nicked at each strand, 12 bp apart, by terminase. This causes linearization of the circular cosmid with two "cohesive" … See more Cosmids are predominantly plasmids with a bacterial oriV, an antibiotic selection marker and a cloning site, but they carry one, or more recently two, cos sites derived from … See more Commonly used vectors include "SuperCos 1" See more • Stryer, Lubert (1995) Biochemistry 4th ed. ISBN 0-7167-2009-4 • Eurekah Biosciences Collection: Viruses, @NCBI See more
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Cosmid - an overview ScienceDirect Topics
WebFeb 1, 1992 · Abstract. Previously, genomic banks of Xanthomonas campestris were constructed in Escherichia coli, using mobilizable broad‐host‐range cosmids as the vectors.Following conjugal transfer, genes involved in the biosynthesis of xanthan polysaccharide (XPS) were cloned by the ability to restore the mucoid phenotype to the … WebA method for preparing an infectious, recombinant virus vector comprises the steps of: (a) infecting host cells with a first virus comprising an encapsidation defective adenovirus (edAd), the edAd comprising a first defective virus genome; (b) incubating the infected host cells in a culture medium for a period of time sufficient for producing infectious virus … fix slow loading web pages on edge windows 11