WebIsobe et al. show that PP1 acts as a RIF1 effector distinct from shieldin in DNA damage repair. RIF1-PP1 inhibits the initiation of resection by CtIP-MRN prior to shieldin action. PP1 and shieldin, as RIF1 effectors, cooperate to suppress premature homologous recombination in the early stage of the damage response. Webresection, and CtIP recruitment and that USP4 interacts with CtIP and MRN. Wijnhoven et al. also showed that such interactions and DDR processes are stimulated by USP4 auto-deubiquitylation on multiple sites, including those on cysteine residues. Wijnhoven et al., 2015, Molecular Cell 60, 362–373 November 5, 2015 ª2015 The Authors
The MRN-CtIP pathway is required for metaphase chromosome
WebVertebrate CtIP, and its fission yeast (Ctp1), budding yeast (Sae2) and plant (Com1) orthologs have emerged as key regulatory molecules in cellular responses to DNA double strand breaks (DSBs). By modulating the nucleolytic 5'-3' resection activity of the Mre11/Rad50/Nbs1 (MRN) DSB repair processing … WebApr 2, 2024 · The domain of CtIP required to promote DNA2 is located in the central region lacking in lower eukaryotes and is fully separable from domains involved in the … dunlavy speakers craigslist
CtIP/Ctp1/Sae2, molecular form fit for function - PubMed
WebJul 13, 2024 · In contrast to the HR-promoting role of the BRCA1-CtIP and MRN-CtIP interactions, the 53BP1-RIF1 complex is known to inhibit the DSB resection process to suppress HR (Bunting et al., 2010; Zimmermann and de Lange, 2014). Following DNA damage, 53BP1 accumulates on DSB sites and is phosphorylated on S/T-Q sites by … WebThe MMEJ pathway inhibitor can be a CtIP or MRN inhibitor. The CtIP inhibitor can be selected from KLHL15 and PIN1. The MRN inhibitor can be selected from E1b55K and E40rf6. [0021] In some embodiments, a first nucleic acid … WebSep 14, 2024 · The C-terminal–binding protein (CtBP)-interacting protein (CtIP), like its yeast ortholog Sae2, initiates DNA end resection together with the MRE11-RAD50-NBS1 (MRN) nuclease complex ().By virtue of its resection activity, CtIP was implicated in A-EJ (7–9).CtIP expression and protein levels are higher in S and G2 phases and lower in the … dunlavy speakers connect