WebImmunofluorescence analysis of Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Cyanine3 ( Product # A10521) was performed using SH-SY5Y (positive model) and T-47D (negative model) cells stained with Nestin Monoclonal Antibody (10C2), eBioscience™ (Product # 14-9843-80). WebSee product label Cy™3 Goat Anti-Rabbit IgG (H+L) ... Cy3 is a suitable replacement for TRITC in multiple-labeling experiments with FITC, though overlap in FITC-Cy3 emmission spectra may necessitate use of narrow band-pass filters. Cy3 may also be paired with CyTM5 for double labeling, due to their well-separated emission peaks. However, Cy5 ...
Donkey Anti-Goat IgG H&L (Cy3 ®) preadsorbed (ab6949)
WebCy3 Goat Anti-Rabbit IgG (H+L) Antibody w kategorii Fluorescencyjnie / Przeciwciała znakowane / Immunologia WebApr 12, 2024 · Prior to washing three times with 0.05% Tween 20/TBS (TBST) over 30 min, the membranes were probed with peroxidase-conjugated AffiniPure goat anti-mouse immunoglobulin G (IgG) (H + L; 1:10,000; Jackson ImmunoResearch, PA, USA) or horseradish peroxidase (HRP)-labeled goat anti-rabbit IgG (H + L; 1:2500; Servicebio, … health benefits of pineapple for females
Cy3® goat anti-mouse IgG (H+L) AAT Bioquest
WebBased on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule rabbit IgG. It also reacts with the light chains of other rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with human ... WebApr 14, 2024 · Each Cy3-labeled sample was combined with an equal amount of a Cy5-labeled sample, and the pooled Cy2-labeled sample was added as an internal standard. ... Alexa FluorTM 594 goat anti-mouse IgG [H+L] (Invitrogen, Waltham, MA, USA) or Alexa FluorTM 488 goat anti-rabbit IgG (Invitrogen, USA) at a dilution of 1:500. To block … WebAfter 48 h of incubation with both of the primary antibodies, sections were washed and incubated with a Cy3 labeled anti-mouse secondary antibody and with donkey anti-rabbit secondary antibody (Abcam, Cambridge, UK; Goat Anti-Mouse IgG H&L (Alexa Fluor ® 488 ab150113), and Goat Anti-Rabbit IgG H&L (Alexa Fluor ® 488, ab150077), 1:300 in ... golf r-line tdi s-a