Fitc sirna
WebAug 21, 2024 · FITC-siRNA (0.4 µg) complexes with the carriers were prepared at various charge ratios. After transfection in serum-free medium for 2 h, KB cells and 786-0-luc cells were supplemented with fresh media containing 10% FBS and incubated for further 22 h. After washing with phosphate-buffered saline (PBS, pH 7.4) twice, the cells were … WebApr 21, 2024 · AuNP-PEI-peptide-FITC could bind siRNA with PEI by electrostatic interactions to form the AuNP-PEI-peptide-FITC/siRNA nanocomplexes, allowing …
Fitc sirna
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WebOct 10, 2024 · Control siRNA (FITC Conjugate)-A siRNA (), shRNA and Lentiviral Particle Gene Silencers designed to knockdown Control gene expression. Cited in 365 publications WebSep 17, 2009 · C, binding of FITC-labeled siRNA to MCF-7 cells in the presence of C9 (black filled peak) or as a complex with C9-P (open grey peak). Cells were incubated on ice with a 4:1 mixture of FITC-labeled siRNA and C9 or C9-P for 1 h. After repeated washing, cell binding was determined by flow cytometry analysis.
WebFluorescein isothiocyanate (FITC)-labeled siRNA (siRNA-FITC) was applied as a reporter gene, and the amount of siRNA was kept at 0.1 nm/well, while the weight ratios of DMC/SiRNA-FITC were 5/1, 25/1, and 50/1, respectively. The fluorescence microscope (Carl Zeiss Meditec AG, Jena, Germany) and flow cytometry (Epics Elite ESP; Beckman … WebFITC-labeled AM and unlabeled AM were synthesized according to the sequencing results and detected by flow cytometry and laser confocal microscope. Preparation of nano-zinc carrier and its coupling with Aptamers. ... To quantify siRNA loaded in nano-zinc carriers, the nano-zinc carriers loaded with USP14 siRNA at various weight ratios were ...
WebFluorescein isothiocyanate (FITC)-labeled siRNA (siRNA-FITC) was applied as a reporter gene, and the amount of siRNA was kept at 0.1 nm/well, while the weight ratios of … WebApr 11, 2024 · Similarly, 4T1 cells were incubated with [email protected] FITC and HPB-S-PP/siRNA FAM for different times to investigate the co-location of LOx FITC or siRNA FAM with lysosome. The cellular uptake of co-delivery NPs was quantitatively analyzed by flow cytometry (Beckman Coulter). Briefly, 4T1 cells were seeded in a six-well plate ...
WebNov 6, 2016 · The incorporation of FITC-siRNA-PEI onto the porous PU films was evaluated by a fluorescence microscope (BX-51, Olympus, Japan), and the release of FITC-siRNA-PEI from the microporous PU films was monitored with a spectrofluorometer (Fluoroskan Ascent FL, Thermo Scientific, USA) at an excitation wavelength of 555 nm and an …
WebOct 10, 2016 · The use of small interference RNA (siRNA) to target oncogenes is a promising treatment approach for cancer. However, siRNA cancer therapies are hindered by poor delivery of siRNA to cancer cells. Transferrin receptor (TfR) is overexpressed in many types of tumor cells and therefore is a potential target for the selective delivery of siRNA … fish electrolysisWebOct 10, 2016 · The use of small interference RNA (siRNA) to target oncogenes is a promising treatment approach for cancer. However, siRNA cancer therapies are … fish electricuted by heaterWebJun 17, 2007 · Figure 3: RVG-9R enables transvascular delivery of siRNA to the central nervous system. a, Mice were injected intravenously with FITC-siRNA/peptide complexes, and uptake by brain, spleen and liver ... fishel engineeringWebcas:464926-03-2 MVL5 一种DNA和siRNA载体. MVL5 是一种不可降解的多价阳离子脂质。. MVL5 是一种高效的 DNA 和 siRNA 载体。. 英文名称：MVL5. 分子量：1164.86. 性状：Oil. 分子式：C59H116Cl5N7O4. canada child benefit in frenchWebAuNP-PEI-peptide-FITC could bind siRNA with PEI by electrostatic interactions to form the AuNP-PEI-peptide-FITC/siRNA nanocomplexes, allowing efficient siRNA delivery in hMSCs. The adipogenic-related gene peroxisome proliferator-activated receptor γ (PPARγ) was targeted for silencing by the AuNP-PEI-peptide-FITC/siRNA nanocomplexes to … canada child benefit income thresholdhttp://www.qiyuebio.com/details/29626 fish electro shockerWebTransfection with Santa Cruz Biotechnology reagents resulted in up to 59% FITC-siRNA positive cells, but did not result in effective silencing of CD80 surface expression. In contrast, the most effective method was the lipid-based method using the siRNA transfection reagent GeneSilencer((R)) from Genlantis. This protocol resulted in up to 92% ... fishel family auto sales