Phis2-p53
Webb12 apr. 2024 · PHIS2-p53+pGAD53m SD-TL SD-TLH 阳性对照 3AT是酵母HIS3蛋白合成的竞争性抑制剂,用于抑制His3基因的泄漏表达。 实验结果如下: 图1:背景筛选结果 结果 … Webb酵母单杂交,p53-His,pLacZ,pHis,pHisi-1,. 产品型号:. 产品时间: 2015-11-08. 可提供近万种质粒载体、菌株、基因和细胞株,并可提供实验技术外包服务,如基因克隆、质粒构建、蛋白原核、真核表达及纯,pQE 载体,原核表达载体,克隆载体 ,毕赤酵母表达载体,毕赤 ...
Phis2-p53
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WebbBait pHIS2.1 containing repeats of your target sequence of interest Prey pGADT7-Rec2 vector containing a library gene Yeast Phenotypes His–, Leu–, or Trp– Requires histidine (His), leucine (Leu), or tryptophan (Trp) in the medium to grow; i.e., is auxotrophic for one (or more) of these specific nutrients Miscellaneous WebbP0153 Mazda HO2S LF Circuit Malfunction. P0153 Mercedes-Benz O2 Sensor Circuit Slow Response Bank 2 Sensor 1. P0153 Mercury O2 Sensor Circuit Slow Response Bank 2 …
Webb9 apr. 2024 · +pHIS2-p53 3 1.2 酵母转化方法 pGAD53m +pHIS2-p53 1. 从YPDA平板挑取Y187单菌落接种于YPDA液体培养基4 ml,30℃,225 rpm,振荡培养18-20 h(过 … Webb14 aug. 2015 · zt028 酵母单杂交载体系统 pHis2 Clontech ... zt1220 pGBK-p53 . zt1221 pGC-silencer-U6-GFP . zt1222 pGIPZ . zt1223 pGL3-FOXO-reporter-luci . zt1224 pGL4.13 . zt1225 pGL4.18 . zt1226 pGL4.19 . zt1227 pGL4.74 . zt1228 pGL5luci . zt1229 ...
Webb实验步骤: 1.构建载体目的基因融合连入pGADT7-Rec2 (AD); 顺式作用元件连入pHis2或pHis2.1载体 (顺式元件可以只连接一个拷贝, 也可以连接3个串联的并测序确认无误)。. 2.验证两载体分别于对应的空载体共转时,有无激活转录报告基因情况,大多酵母苗株有毒 …
WebbVector database is a digital collection of vector backbones assembled from publications and commercially available sources. This is a free resource for the scientific community … china postal customer service numberhttp://www.coolaber.com/Column.asp?Model=Content_Detail&Column_ID=29596&C_ID=284549825 china possible impact geographic featuresWebbFRFS-0353-2 For Epcos saw filter P/N B4959 REV 1.00 Electro-Photonics LLC reserves the right to modify drawings, configurations, specifications, and design at any time without … china post after shippingWebb18 juli 2006 · We generated yeast one-hybrid reporter plasmid by cloning the 356-bp p53 promoter fragment into the pHIS2 reporter plasmid (pHIS2-p53 prom.) and then cotransformed the yeast strain Y187 cells with pHIS2-p53-prom. reporter plasmid and pGAD-Rec2-p53 encoding p53-GAL4 AD fusion. We used pHIS2-p53 cons. reporter … gramlight 57crWebbp53-His2. Catalog No. PVT11251 Packing . p53-His2 Information. Function yeast Hybrid plasmid Resistance Kan Screen / Strain DH5alpha Culture temperature 37degrees … gramlich \\u0026 associates floridagramlick and son funeral home columbus ohioWebb1 dec. 2024 · The pHIS2-p53/pGADT7-Rec2-p53 and pHIS2-p53/pGADT7-Rec2-PagWRKY75 co-transformed yeast cells were used as positive and negative control, respectively. 3.5. Generation of overexpression or knockdown plants for PagWRKY75. gram lights 57c6 time attack edition