Phleomycin plates
WebbPhleomycin-resistant transformants are selected with 10-50 µg/ml of phleomycin in the regeneration medium, depending on the sensitivity of the host strain. Selectivity can be … Webb10 jan. 2014 · Wild type A3-2, an A3-2 mutant carrying phleomycine resistance under control of the constitutive pcbC promoter (C), and three independent xyl1 P-phleo …
Phleomycin plates
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Webb1 jan. 2012 · GYE agar plates containing 3 μg/mL of phleomycin (GYE-Phl): Prepare and autoclave 500 mL GYE agar as described in Subheading 2, item 1. Add 0.5 mL of 3 mg/mL phleomycin stock solution into the warm GYE agar and dispense 10 or 20 mL into each 60- × 15-mm or 100- × 15-mm Petri plate, respectively. WebbBleomycin and phleomycin are closely related broad-spectrum antibiotics produced by strains of Streptomyces verticillus; they are effective against both prokaryotic and …
Webb30 nov. 2015 · Plates were photographed after 2 to 3 days. (E) The indicated strains were cultured in the presence of the indicated concentrations of phleomycin for 2 hours, diluted and plated Webb1 maj 1996 · agar plates in the absence of phleomycin for more than. 18 months and retain resistance to the drug at 2 l g/ml. DNA was isolated from the six Pm R transformants …
Webb2 dec. 2010 · PDF Sectoring patterns of mutants in the rad52 epistasis group. Top panels: 5ORIΔ-ΔR chromoductants of rad50Δ, rad51Δ, rad52Δ, rad54Δ, rad55Δ, rad57Δ,... Find, … WebbPhleomycin-resistant transformants are selected in Low Salt LB agar medium (yeast extract 5 g/L, Tryptone 10 g/L, NaCl 5 g/L, Agar 15 g/L, pH7.5) supplemented with 5 …
Webb19 sep. 2013 · The pretreated MA plates were prepared by adding either 50 or 100 µg of Phleomycin (from a 20mg/mL stock) drop wise to each plate in three different regions. After allowing the antibiotic to dry (c.a., 30 min), the individual strains were plated across an entire plate. Resistance was evaluated after two days of growth at either 25°C or 13°C.
Webb29 dec. 2008 · After mixing, regenerated protoplasts were spread on square plates containing 40 ml of solidified MM. Regeneration medium contained no antibiotic, 25 μg of phleomycin ml −1, or 10 μg of nourseothricin ml −1. Selection plates were incubated at 30°C for 5 days. Isolation of genomic DNA and RNA. can i use bitcoin to buy thingsWebbTypically, 100 mm round plates are for growth/selection. ... Produced by Streptomyces verticullus and part of the structurally related group of Bleomycin/Phleomycin type … can i use bisto instead of beef stockWebbFig.2 Phleomycin test plates (1-3: 0, 25, 50 ug/mL) As the images shown above, the growing situation of wild type strain M. anisopliae on hygromycin or phleomycin plates … five oaks nursery \u0026 tree farmWebbThe working concentration of phleomycin for mammalian cell lines varies from 5 to 50 μg/ml. In a starting experiment we recommend to determine the optimal concentration … five oaks new market alabamaWebb1 jan. 2012 · Invert plates after 30 min and place at 37°C (hygromycin); or RT overnight, followed by 30°C (phleomycin, see Note 12). 3. After 24–72 h, ensure the viability of the protoplasts and the validity of the transformation by inspecting, respectively, the “no-drug” control plates, and the positive and negative control plates. 4. can i use biscuit dough for empanadasWebbThe ble marker . The ble gene originates from the tallysomycin-producing actinomycetes species Streptoalloteichus hindustanus and encodes a small (13.7 kDa) protein … five oaks modular homesWebbPhleomycin-resistant transformants are selected with 10-50µg/mL of phleomycin in the regeneration medium, depending on the sensitivity of the host strain. Selectivity can be … can i use bitdefender on iphone