Phospho mtor ser2448

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August undefined, 2024

WebPhosphoPair mTOR (Ser2448) Antibody Set. Whole cell extracts (15 µg protein) from serum starved HeLa and NIH/3T3 cells treated with 10 µM rapamycin (Rap) for 2 hours or with … WebSer2448 phosphorylation is measured to assess mTOR kinase activation or as a measurement of induction of the mTOR pathway in muscle tissue and is implicated in mTORC1 activity [34,37,38,39]. The effects of SLC38A9 and SLC36A1 on mTOR phosphorylation at Ser2448 in C2C12 cells remains unclear.

Phospho-mTOR (Ser2448) Monoclonal Antibody …

WebPhospho-mTOR (S2448) and Total mTOR ELISA Kit (ab279869) is a very rapid, convenient, and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated mTOR protein in your experimental model system, you can verify pathway activation in your cell lysates. WebmTOR (phospho Ser2448) stained by mTOR (phospho Ser2448) antibody (GTX132803) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min GTX132803 WB Image Untreated (–) and treated (+) 293T whole cell extracts (60 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with mTOR (phospho Ser2448) antibody … small zip bags wholesale

Phospho-mTOR (Ser2448) (49F9) Rabbit mAb (IHC Specific) Cell Sign…

WebJan 9, 2024 · Both mTORC1 and mTORC2 are regulated by phosphorylation, complex formation and localization. So far, the role of p70S6K-mediated mTOR S2448 phosphorylation has not been investigated in detail. Here, we report that endogenous mTOR phosphorylated at S2448 binds to both, raptor and rictor. WebPhospho-mTOR (Ser2448) Antibody (25-9718-42) in Flow Normal human peripheral blood cells were unstimulated (left) or stimulated with Anti-Human CD3 and CD28 Functional … WebPrimary antibodies for immunohistochemistry (IHC) included anti-mTOR, anti-phospho-mTOR Ser2448, anti-S6 ribosomal protein, anti-Beclin1 (all from Abcam, Cambridge, UK) … small zipless cooler

Phospho mTOR (Ser2448) cellular HTRF kit Cisbio

Phospho-mTOR (Ser2448) Recombinant Rabbit Monoclonal …

Webresults in elevated mTOR activity in vitro and in cells (18, 20). In this study, we demonstrate that mTOR phosphorylation at Ser-2448 is blocked by rapamycin, and this effect is inde-pendent of the AKT activation status, which suggests that Ser-2448 phosphorylation is catalyzed by a protein kinase other than AKT. We show that Ser-2448 is ... WebWe show that Ser-2448 is directly phosphorylated byp70S6 kinase in vitro and that expression of a rapamycin-resistantp70S6 kinase in cells maintains Ser-2448 phosphorylation in the presence ofrapamycin. Furthermore, changes in amino acid availability, which affectsp70S6 kinase activity, also modulates Ser-2448 phosphorylation. hilary prosserWebPhosphoPlus® mTOR (Ser2448) Antibody Duet PhosphoPlus ® mTOR (Ser2448) Antibody Duet #39182 Reviews () Citations (2) Flow cytometric analysis of A549 cells using mTOR … hilary probiotics

"WebPhospho-mTOR (Ser2448) Antibody (46-9718-42) in Flow TOP: Normal human peripheral blood cells were unstimulated (left) or stimulated with Anti-Human CD3 Functional Grade … " - Phospho mtor ser2448

Phospho mtor ser2448

Phosphorylation of Mammalian Target of Rapamycin (mTOR) …

WebAnti-phospho-mTOR (Ser2448) Antibody Upstate®, from rabbit; Synonyms: FRAP1, FRAP, FRAP2, RAFT1, RAPT1, FK506 binding protein 12-rapamycin associated protein 1; find … WebmTOR (Mammalian Target of Rapamycin, aka FRAP, RAPT or RAFT) is a large 289 kDa Ser/Thr protein kinase that regulates cell cycle progression, cell growth, protein synthesis, ribosome biogenesis, and autophagy. mTOR is an evolutionarily conserved member of the Phosphoinositol Kinase-related Kinase (PIKK) family whose activity is regulated by …

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WebThe MSD Phospho(Ser2448)/Total mTOR Assay is available on 96-well 4-Spot plates. This datasheet outlines the performance of the assay. Typical Data Representative results for … WebRayBio® Human and Mouse Phospho-mTOR (Ser2448) and Total mTOR ELISA Kit. This assay semi-quantitatively measures mTOR phosphorylated at Serine-2448 as well as total mTOR in cell lysate samples. $567.00 CODE: PEL-mTOR-S2448-T-1 Number of Plates: Quantity: + − Add To Cart View all product options Description Reviews Ask a question …

WebMonoclonal Antibody for studying mTOR (Ser2448) phosphate. Validated for Immunohistochemistry (Paraffin). Highly specific and rigorously validated in-house, … WebThe MSD Phospho(Ser2448)/Total mTOR Assay is available on 96-well 4-Spot plates. This datasheet outlines the performance of the assay. Typical Data Representative results for the Phospho(Ser2448)/Total mTOR Assay are illustrated below. The …

WebProduct Description. The PathScan ® Phospho-mTOR (Ser2448) Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects … WebPhospho-mTOR (Ser2448) (49F9) Rabbit mAb (BSA and Azide Free) detects endogenous levels of mTOR protein only when phosphorylated at Ser2448. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser2448 of human …

WebJan 1, 2024 · Starting on day 15, mice were treated three times weekly with 1 mg/tumor Mito-MGN administered via an intratumoral injection in a 50 μL volume, and the control mice were injected with a vehicle of 20% ethanol in phosphate buffered saline.

WebApr 5, 2024 · Several upstream signals, including ERK, converge on the phosphorylation and inhibition of Tuberous Sclerosis Complex (TSC) to activate mTORC1 [20, 21]. mTOR functions as the catalytic subunit of two individual complexes, known as … hilary proppWebFeb 12, 2024 · We measured the levels of mTOR phosphorylation at Ser2448, and the mTOR target protein 49, the ribosomal protein S6 kinase (p70S6K) phosphorylation at Thr389, which indicates the mTORC1... hilary proutyWebThe mTOR subunit of mTORC1 and 2 is phosphorylated at Ser2448 in a PI3/Akt- signaling-dependent manner; this post-translational modification is associated with increased … small zero wall reclinerWebmTOR抑制剂的筛选方法. 分析：OD值越小，mTOR的Ser2448磷酸化程度越低，抑制剂作用越强。. （2）荧光共振能量转移（FRET）. 荧光共振能量转移是指两个荧光发色基团在足够靠近（7~10nm）时，当供体分子吸收一定频率的光子后被激发到更高的电子能态，在该电子回 … small zip ties clearWeb斯氏毛滴虫pcr检测试剂盒厂家上海联祖生物相关产品:28s rrnp检测试剂盒实验流程1.实验前标准品、试剂及样本的准... small zip lock bag sizeWebNov 7, 2015 · Antibodies against total phospho-AMPK(Thr172), total phospho-ACC(Ser79), total phospho-p53(Ser15), phospho-Raptor (Ser722/ Ser792), total phospho-mTOR(Ser2448), total phospho-p70S6K(Thr389), total phospho4EBP-1 (Thr 37/46), total phospho-Akt(Ser 473), LC3 I/II β-actinwere obtained fromCell Signaling Technology, … hilary pronounceWebMammalian target of rapamycin (mTOR) is a serine/threonine kinase that plays a key role in cell growth, cell proliferation, and protein synthesis. mTOR mediates phosphoinositide 3-kinase and Akt/PKB signaling, resulting in phosphorylation … hilary pritchard obituary 1996