WebJan 2, 2024 · Puromycin dihydrochloride is the dihydrochloride salt of puromycin. Puromycin is an aminoglycoside antibiotic that inhibits protein synthesis. Puromycin blocks protein synthesis after aminoacyl-sRNA formation, and at the same time it leads to the accumulation of small peptides. Both of these effects appear to be due to the splitting of …
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WebJan 4, 2024 · OP-Puro is an analog of puromycin that contains a terminal alkyne group 6.Similar to puromycin, OP-Puro is an aminoacyl-tRNA analog that enters the acceptor site of ribosomes, is incorporated into ... WebApr 30, 2014 · Point mutations in EBC5-16 were generated by using Quick Change (Agilent) site-directed mutagenesis using codon-optimized EBC5-16 cloned into the retroviral vector, pMSCV-puro (Clontech), as the starting template. pRVY-hygro/TC2-3 and pRVY-hygro/EBC5-16 were generated by cloning TC2-3 and EBC5-16, respectively, from pRVY … guide to selling a house
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